■ 基本信息

■ 質(zhì)粒屬性

■ 質(zhì)粒簡介

pFastBac1載體是非融合性蛋白表達載體，在載體內(nèi)部不帶有其他融合性的蛋白標簽。為了保證目的基因的正確表達，插入片段必須包含ATG起始密碼子，保證蛋白起始翻譯。插入片段最好包含終止密碼子，保證蛋白質(zhì)不會翻譯出多余的氨基酸。在pFastBacI載體多克隆位點中，對于所有的三種讀碼框分布，都包含有終止密碼子。

The Bac-to-Bac? Vector Kit contains a pFastBac? 1 vector, as well an expression control vector, intended for use as part of the Bac-to-Bac? Baculovirus Expression System , which enables the efficent production of recombinant baculovirus for expression testing in insect cells. The Bac-to-Bac? System relies on generation of recombinant baculovirus by site-specific transposition in E. colirather than homologous recombination in insect cells (Figure 1). This system features:

Time-saving expression bacmid. With Bac-to-Bac?, the expression cassette of the pFastBac? vector recombines with the parent bacmid in DH10Bac? E. coli Competent Cells (not included with this vector kit) to form an expression bacmid. The bacmid is then transfected into insect cells for production of recombinant baculovirus particles.

Easy colony screening. The parent bacmid in DH10Bac? E. colicontains a segment of the lacZα gene. The lacZα gene is disrupted upon transposition of the expression cassette into the bacmid allowing for blue/white selection of recombinants. This makes identification  of recombinant colonies easy.

■ 質(zhì)粒圖譜

■ 質(zhì)粒序列

質(zhì)粒序列請下載：ZK1037pFastBac1昆蟲胞內(nèi)質(zhì)粒.txt

質(zhì)粒只保證關(guān)鍵序列正確，不保證表達效果。