■ 基本信息
復(fù)制子: | pUC |
終止子: | SV40 poly(A) signal |
質(zhì)粒分類(lèi): | 哺乳細(xì)胞，哺乳細(xì)胞雙雜交載體 |
質(zhì)粒大小: | 4955bp |
原核抗性: | Amp |
克隆菌株: | DH5a |
培養(yǎng)條件: | 37度 |
表達(dá)宿主: | 哺乳細(xì)胞 |
誘導(dǎo)方式: | 無(wú)須誘導(dǎo)，瞬時(shí)表達(dá) |
5'測(cè)序引物: | Sv40-polyA-R：GAAATTTGTGATGCTATTGC |
3'測(cè)序引物: | 根據(jù)序列設(shè)計(jì)引物 |
■ 質(zhì)粒屬性
質(zhì)粒宿主: | 哺乳細(xì)胞 |
質(zhì)粒用途: | 雜交 |
片段類(lèi)型: |
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片段物種: |
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原核抗性: | Amp |
真核抗性: |
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熒光標(biāo)記: |
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■ 質(zhì)粒簡(jiǎn)介
The pG5luc Vector contains five GAL4 binding sites upstream of a minimal TATA box, which in turn is upstream of the firefly luciferase gene. The pGAL4 and pVP16 fusion constructs are transfected along with the pG5luc Vector into mammalian cells. Two to three days after transfection, the cells are lysed, and the amount of Renilla luciferase and firefly luciferase are quantitated using the Dual-Luciferase Reporter Assay System. Interaction between the two test proteins, expressed as GAL4 and VP16 fusion constructs, results in an increase in firefly luciferase expression as compared to the negative controls.
■ 質(zhì)粒圖譜
■ 質(zhì)粒序列
質(zhì)粒序列請(qǐng)下載：
ZK549pG5Luc哺乳雙雜交質(zhì)粒.txt
